Detection of hdc Gene from Histamine Producing Bacillus subtillis and Serratia marcescens

作者: C. Aishwarya Laxmi , S. Munaliga , N.G. Ramesh Babu , R. Ragunathan

DOI: 10.3126/IJASBT.V5I3.17826

关键词:

摘要: In the present study, two histamine producing bacteria were used to detect presence of hdc gene using PCR. The production was confirmed by Soyabean Casein Digest Agar (Tryptone Soya Agar). DNA isolated Phenol: chloroform method. PCR carried out and amplified product (657bps 668 bps respectively). Further, transformed into E.coli transformation confirmed.Int. J. Appl. Sci. Biotechnol. Vol 5(3): 309-312

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