New monoclonal anti-mouse DC-SIGN antibodies reactive with acetone-fixed cells

摘要: Mouse DC-SIGN CD209a is a type II transmembrane protein, one of family C-type lectin genes syntenic and homologous to human DC-SIGN. Current anti-mouse monoclonal antibodies (MAbs) are unable react with in acetone-fixed cells, limiting the chance visualize tissue sections. We first produced rabbit polyclonal PAb-DSCYT14 against 14-aa peptide cytosolic domain mouse DC-SIGN, it specifically detected not related lectins, SIGN-R1 SIGN-R3 expressed transfected CHO cells. MAbs were generated by immunizing rats knockout mice extracellular region Five rat IgG2a or IgM MAbs, named BMD10, 11, 24, 25, 30, selected each MAb FACS Western blots, although BMD25 was cross-reactive SIGN-R1. Two IgG2c MMD2 MMD3 interestingly bound but at 10 fold higher levels than MAbs. When binding epitopes new BMD two other commercial anti-DC-SIGN 5H10 LWC06, examined competition assays, BMD11, LWC06 identical closely overlapping while shown bind different epitopes. on 3rd noncompeting cell surface sensitive collagenase treatment, as monitored MAb. These reagents should be helpful probe biology vivo.