Protein identification using 20-minute Edman cycles and sequence mixture analysis.
作者： William J. Henzel , Jake Tropea , David Dupont
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摘要: We have developed a 20-min Edman cycle and multiple sample horizontal flow reactor for the sequence analysis of PVDF-electroblotted proteins. The uses 12-min C18 phenylthiohydantoin separation. This separation is compatible with most Applied Biosystems sequencers. Using this rapid cycle, 10 residues on six different proteins can be completed within 24-h period. also demonstrate use an algorithm that sort mixture sequences derived from PVDF bands contain coeluting
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