A mineral sunscreen affords genomic protection against ultraviolet (UV) B and UVA radiation: in vitro and in situ assays.
作者： Cayrol , Sarraute , Tarroux , Redoules , Charveron
DOI: 10.1046/J.1365-2133.1999.02973.X
关键词:
摘要: Ultraviolet (UV) radiation has been shown to be responsible for different biological effects on human skin, including the initiation of photocarcinogenesis. Both UVB and UVA have described as mutagenic, but processes by which they alter DNA are different. Although cells can repair damage, some deleterious mutations nevertheless appear promote cancer. The risk photocarcinogenesis is acknowledged frequency photogenodermatosis increasing. In order evaluate protection efficacy a high sun factor (SPF) mineral sunscreen against UVB- UVA-induced genomic alterations, we followed two approaches. First, tested its ability decrease unscheduled synthesis response in vitro fibroblasts, an indirect measure UVB-induced lesions (0.005 0.01 J/cm2), second, verified reduce situ end-labelling intensity skin direct single-strand breaks (10 J/cm2). Microscopic analysis clearly demonstrated protective effect UVA. A dose-dependent sunscreens was observed. There also relationship between SPF protection. By limiting accumulation UV-induced DNA, this could limit mutation frequency.
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