Shotgun DNA microarrays and stage-specific gene expression in Plasmodium falciparum malaria.

摘要: Summary Malaria infects over 200 million individuals and kills 2 young children every year. Understanding the biology of malarial parasites will be facilitated by DNA microarray technology, which can track global changes in gene expression under different physiological conditions. However, genomes Plasmodium sp. (and many other important pathogenic organisms) remain to fully sequenced so, currently, it is not possible construct gene-specific microarrays representing complete genomes. In this study, 3648 random inserts from a falciparum mung bean nuclease genomic library were used shotgun microarray. Through differential hybridization sequencing relevant clones, large differences identified between blood stage trophozoite form parasite sexual gametocyte form. The present study lengthens our list stage-specific transcripts malaria at least an order magnitude above all previous studies combined. results offer unprecedented number leads for developing transmission blocking agents vaccines directed antigens. A significant fraction stage-selective had no sequence homologues current genome data bases, thereby underscoring importance approach. useful unravelling additional aspects general approach may applied any organism, regardless how much its sequenced.