Study of triacylglycerol assembly by Apiotrichum curvatum ATCC 20509 as a model system

摘要: The oleaginous yeast Apiotrichum curvatum was grown on various lipids as carbon sources. When commercial animal and vegetable oils were used substrates, the triglyceride had a fatty acid profile similar to that of substrate oil, but glyceride structures altered. Saturated free acids with less than 14 carbons could not be sources by yeast. An emulsion palmitic well utilized yeast, it extensively desaturated before being deposited in triglyceride. Stearic arachidic emulsions gave very limited no growth, respectively. Oleic supported good growth; eicosenoic erucic poor growth. grew petroselinic its supplemented 1000 ppm butylated hydroxyanisole, linoleic linolenic showed excellent growth lipid accumulation. ricinoleic acid, eleostearic vernolic when triglycerides containing these acids, contained conjugated oxygenated found oils. crambe oil source for 20 or more concentrated residual while those 16 18 almost completely utilized. cholesterol incorporated medium, little into depot fats.