[63] Ion-exchange chromatography of citric acid cycle components and related compounds

摘要: Publisher Summary This chapter discusses the Ion-exchange chromatography of citric acid cycle components and related compounds that were first applied in biochemical studies formate a formic gradient to elute organic acids. The variants this procedure include use ammonium formate-formic acetate columns with an acetic gradient. These procedures are used widely separate appreciable amounts removal large or acids, their salts, is time-consuming, it not well adapted for either separation nanomole quantities simultaneous monitoring radioactivity where isotopic acids employed. Quantities greatly excess 5 micromoles individual alter pH lead peak distortion displacement both. Where certain together (overlap), chemical reduction permits rapid second using same chromatographic system. For example, citrate pyruvate overlap, while suecinate acetoacetate, fumarate a-ketoglutarate as single peaks. techniques described useful studying dynamic aspects metabolism mitochondrial systems.