Preventing PCR Amplification Carryover Contamination in a Clinical Laboratory

作者: Jaber Aslanzadeh

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摘要: During the past two decades PCR and several other DNA/RNA amplification techniques have become important diagnostic tools in clinical laboratories. Amplification products contamination has been main impediment to using these routinely Over years, creative pre- post-amplification methods developed that prevent amplicon carryover contamination. These procedures, coupled with automated systems employ real-time simultaneous detection a closed system, substantially reduced possibility of false positive results due

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