Cloning and characterization of the HLIP gene encoding high light-inducible protein from Porphyra yezoensis

作者: Fanna Kong , Yunxiang Mao , Hui Yang , Li Wang , Limin Liu

DOI: 10.1007/S10811-011-9686-Z

关键词:

摘要: High light-inducible proteins are critical for photosynthetic organisms when responding to high light stress. We cloned and characterized the HLIP gene from Porphyra yezoensis, which was designated as PyHLIP. Sequence analysis revealed that open reading frame of PyHLIP 387 bp in length encoded a 128 amino acid-polypeptide containing plastid transit peptide an obvious membrane-spanning α-helix region. No intron existed genomic DNA The putative promoter TATA-less, initiator element (Inr) upstream ATG. Analysis organization indicated there were two copies P. yezoensis nuclear genome. expression can be induced by various stress conditions is especially sensitive light.

参考文章(36)
Kong FanNa, Mao YunXiang, Cui JingJing, Construction of genomic fosmid library of Porphyra yezoensis. Journal of Fisheries of China. ,vol. 33, pp. 396- 402 ,(2009)
Stefan Jansson, Jenny Andersson, Soo Jung Kim, Grzegorz Jackowski, An Arabidopsis thaliana protein homologous to cyanobacterial high-light-inducible proteins Plant Molecular Biology. ,vol. 42, pp. 345- 351 ,(2000) , 10.1023/A:1006365213954
Qingfang He, Nadia Dolganov, Olle Björkman, Arthur R. Grossman, The high light-inducible polypeptides in Synechocystis PCC6803. Expression and function in high light. Journal of Biological Chemistry. ,vol. 276, pp. 306- 314 ,(2001) , 10.1074/JBC.M008686200
Werner Kühlbrandt, Da Neng Wang, Yoshinori Fujiyoshi, Atomic model of plant light-harvesting complex by electron crystallography Nature. ,vol. 367, pp. 614- 621 ,(1994) , 10.1038/367614A0
Michael Reith, Janet Munholland, Complete nucleotide sequence of thePorphyra purpurea chloroplast genome Plant Molecular Biology Reporter. ,vol. 13, pp. 333- 335 ,(1995) , 10.1007/BF02669187
Maureen L Coleman, Matthew B Sullivan, Adam C Martiny, Claudia Steglich, Kerrie Barry, Edward F DeLong, Sallie W Chisholm, None, Genomic islands and the ecology and evolution of Prochlorococcus Science. ,vol. 311, pp. 1768- 1770 ,(2006) , 10.1126/SCIENCE.1122050