Operation of a benchtop bioreactor.

作者: Kristina M. Obom , Andrew Magno , Patrick J. Cummings

DOI: 10.3791/50582

关键词:

摘要: Fermentation systems are used to provide an optimal growth environment for many different types of cell cultures. The ability afforded by fermentors carefully control temperature, pH, and dissolved oxygen concentrations in particular makes them essential efficient large scale expression fermentation products. This video will briefly describe the advantages fermentor over shake flask. It also identify key components a typical benchtop system give basic instruction on setup vessel calibration its probes. viewer be familiarized with sterilization process shown how inoculate medium culture. Basic concepts operation, sampling, harvesting demonstrated. Simple data analysis cleanup discussed.

参考文章(6)
Matthew DeLisa, Michael L. Shuler, Fikret Kargı, Bioprocess Engineering: Basic Concepts ,(1991)
Zhenyu Wang, Mellisa Ly, Fuming Zhang, Weihong Zhong, Amy Suen, Anne Marie Hickey, Jonathan S. Dordick, Robert J. Linhardt, E. coli K5 fermentation and the preparation of heparosan, a bioengineered heparin precursor. Biotechnology and Bioengineering. ,vol. 107, pp. 964- 973 ,(2010) , 10.1002/BIT.22898
S. Jana, J. K. Deb, Strategies for efficient production of heterologous proteins in Escherichia coli. Applied Microbiology and Biotechnology. ,vol. 67, pp. 289- 298 ,(2005) , 10.1007/S00253-004-1814-0