Assessment of clonal fidelity of micropropagated gerbera plants by ISSR markers

摘要: Abstract True-to-type clonal fidelity is one of the most important pre-requisites in micropropagation crop species. Genetic vitro raised 45 plants gerbera (Gerbera jamesonii Bolus) derived from three different explants, viz., capitulum, leaf and shoot tips, was assessed by 32 ISSR markers, for their genetic stability. Out 15 markers produced clear, distinct scorable bands with an average 5.47 per marker. The designed AG motif amplified more number bands. anchored at 3′ ends high consistent than unanchored markers. Fifteen generated a total 3773 bands, out which 3770 were monomorphic among all clones. Jaccard's similarity coefficient revealed that clones 44 grouped into single large cluster alongwith mother plant value 1.00, whereas clone (C38) remained ungrouped. capitulum tip explants did not show any variation, whereas, leaf-derived exhibited some degree variation.