Analysis of Block of cell proliferation 1 (BOP1) activity in strawberry and Arabidopsis.

摘要: Block of cell proliferation (BOP) proteins are conserved among eukaryotes, and studies in mammals yeast have described their role ribosome biogenesis cycle regulation. A BOP1 orthologue was identified plants, loss-of-function analyses tobacco cells confirmed similar activities. This report characterizes a for activity planta. Two transgenic plant species were used: the diploid strawberry (Fragaria vesca) Arabidopsis thaliana. FvBOP1 silencing showed changes pre-rRNA processing, demonstrated FvBOP1's growth physiology throughout different stages development. In strawberry, repression decreased fitness prior to flowering, followed by death after reproductive transition, indicating that is required transition back vegetative flowering. T-DNA null allele AtBOP1 gene lethal, 50% decrease transcript accumulation sufficient cause severe developmental defects linked defective division. The protein essential viability. Lower levels result rRNA processing abnormalities consistent with its predicted biogenesis.