Essential RNA binding and packaging domains of the Gag-Pol fusion protein of the L-A double-stranded RNA virus of Saccharomyces cerevisiae.

摘要: The crucial process in the assembly of L-A double-stranded RNA virus is recognition its (+) single-stranded by Gag-Pol protein. Pol region this protein has binding activity and necessary for packaging. Here we show that there are actually two vitro RNA-binding domains (residues 172-190 770-819), both viral propagation, (but not particle assembly). Furthermore, N-terminal domain vivo packaging RNA. We precisely define extent 67-213), which includes domain. This suggests responsible genomic additional surrounding residues specificity binding.