Real-time 2D visualization of metabolic activities in zebrafish embryos using a microfluidic technology.

摘要: Non-invasive and real-time visualization of metabolic activities in living small model organisms such as embryos larvae zebrafish has not yet been attempted largely due to profound analytical limitations existing technologies. Historically, our capacity examine oxygen gradients surrounding eggs severely limited, so much that date, most the articles characterizing situ have described predominantly mathematical simulations. These drawbacks can, however, be experimentally addressed by an emerging field microfluidic Lab-on-a-Chip (LOC) technologies combined with sophisticated optoelectronic sensors. In this work, we outline a proof-of-concept approach utilizing embryo array system enable Fluorescence Ratiometric Imaging (FRIM) on developing embryos. The FRIM is innovative method for kinetic quantification temporal patterns aqueous at very fine scale based signals coming from optical sensor referred foil. We envisage future integration chip-based represents noteworthy direction miniaturize revolutionize research metabolism physiology vivo.