Proteome and Phosphoproteome Changes Associated with Prognosis in Acute Myeloid Leukemia

摘要: Acute myeloid leukemia (AML) is a hematological cancer that mainly affects the elderly. Although complete remission (CR) achieved for majority of patients after induction and consolidation therapies, nearly two-thirds relapse within short interval. Understanding biological factors determine has become major clinical interest in AML. We utilized liquid chromatography tandem mass spectrometry (LC-MS/MS) to identify protein changes phosphorylation events associated with AML primary cells from 41 at time diagnosis. Patients were defined as relapse-free if they had not relapsed five-year follow-up Relapse was increased expression RNA processing proteins decreased V-ATPase proteins. also observed an increase catalyzed by cyclin-dependent kinases (CDKs) casein kinase 2 (CSK2). The relevance proteome findings supported cell proliferation assays using inhibitors (bafilomycin), CSK2 (CX-4945), CDK4/6 (abemaciclib) CDK2/7/9 (SNS-032). While bafilomycin preferentially inhibited patients, less efficient these cells. This suggests therapy against upregulated could target inducing their upregulation rather than activity. study, therefore, presents markers help predict direct therapeutic strategies.