Methylation of multiple genes in hepatitis C virus associated hepatocellular carcinoma

摘要: We studied promoter methylation (PM) of 11 genes in Peripheral Blood Lymphocytes (PBLs) and tissues hepatitis C virus (HCV) associated hepatocellular carcinoma (HCC) chronic (CH) Egyptian patients. The present study included 31 HCC with their ANT, 38 CH 13 normal hepatic tissue (NHT) samples. In all groups, PM APC, FHIT, p15, p73, p14, p16, DAPK1, CDH1, RARβ, RASSF1A, O6MGMT was assessed by methylation-specific PCR (MSP). APC O6-MGMT protein expression immunohistochemistry (IHC) the (20 samples each) as well a different set for confirmation MSP results. progression from to HCC. Most showed high frequency (MF) index (MI) increased disease progression. MF CDH1 significantly higher ANT. CH. reported concordance between PBL O6MGMT. A panel 4 (APC, O6MGMT) classifies cases independently into accuracy (89.9%), sensitivity (83.9%) specificity (94.7%). HCV infection may contribute hepatocarcinogenesis through enhancing multiple genes. occurs early cascade while RARB, are late changes. could be used monitoring patients detection Also, we found that, status is not affected whether liver or PBL, indicating possibility use indicator genetic profile instead regardless stage disease.