Grape Powder Supplementation Attenuates Prostate Neoplasia Associated with Pten Haploinsufficiency in Mice Fed High-Fat Diet.

摘要: Scope Previous studies have identified potent anticancer activities of polyphenols in preventing prostate cancer. The aim the current study is to evaluate chemopreventive potential grape powder (GP) supplemented diets genetically predisposed and obesity-provoked Methods results Prostate-specific Pten heterozygous (Pten+/f ) transgenic mice are fed low- high-fat diet (LFD HFD, respectively) with 10% GP for 33 weeks, ad libitum. Prostate tissues characterized using immunohistochemistry western blots, sera analyzed by ELISA qRT-PCR. Pten+/f LFD HFD show favorable histopathology, significant reduction proliferative rate epithelial cells (Ki67), rescue PTEN expression. most protective effect supplementation detected against HFD-induced increase inflammation (IL-1β; TGF-β1), activation cell survival pathways (Akt, AR), angiogenesis (CD31) mice. Moreover, reduces circulating levels oncogenic microRNAs (miR-34a; miR-22) There no changes body weight food intake groups. Conclusions can be a beneficial strategy obesity-related cancer progression. Monitoring serum miRNAs facilitate non-invasive evaluation chemoprevention efficacy.