Inactivation of thyrotropin-releasing hormone (TRH) and (3Me-HIS) TRH by brain peptidases studied by high-performance liquid chromatography
作者： E.C. Griffiths , J.R. McDermott , A.I. Smith
DOI: 10.1016/0304-3940(82)90209-9
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摘要: High-performance liquid chromatography (HPLC) has been used to separate and identify the metabolites formed from thyrotrophin-releasing-hormone (TRH) its hyperactive analogue, (3Me-His)TRH, by subcellular fractions rat cortex, hypothalamus thalamus. Deamidation proline endopeptidase formation of histidylproline diketopiperazines pyroglutamyl aminopeptidase were found be major mechanisms brain inactivation both peptides; (3Me-His)TRH was slightly more stable than TRH in presence peptidases, with enhanced receptor binding affinity, this could explain increased biological activity. The HPLC system may applicable determining other analogues also define pathways for larger neuropeptides as well.
sci-hub.se 参考文章(14)
C. Prasad, A. Peterkofsky, T. Matsui, Metabolism of thyrotropin releasing hormone in brain extracts. Isolation and characterization of an imidopeptidase for histidylprolineamide. Journal of Biological Chemistry. ,vol. 254, pp. 2439- 2445 ,(1979) , 10.1016/S0021-9258(17)30242-9
T. Yanagisawa, C. Prasad, A. Peterkofsky, The subcellular and organ distribution and natural form of histidyl-proline diketopiperazine in rat brain determined by a specific radioimmunoassay. Journal of Biological Chemistry. ,vol. 255, pp. 10290- 10298 ,(1980) , 10.1016/S0021-9258(19)70463-3
C Prasad, A Peterkofsky, Demonstration of pyroglutamylpeptidase and amidase activities toward thyrotropin-releasing hormone in hamster hypothalamus extracts. Journal of Biological Chemistry. ,vol. 251, pp. 3229- 3234 ,(1976) , 10.1016/S0021-9258(17)33427-0
Alan Peterkofsky, Fiorenzo Battaini, The biological activities of the neuropeptide histidyl-proline diketopiperazine Neuropeptides. ,vol. 1, pp. 105- 118 ,(1980) , 10.1016/0143-4179(80)90014-1
John E. Morley, Extrahypothalamic Thyrotropin Releasing Hormone (TRH) — Its distribution and its functions Life Sciences. ,vol. 25, pp. 1539- 1550 ,(1979) , 10.1016/0024-3205(79)90435-1
John H. Rupnow, William L. Taylor, Jack E. Dixon, Purification and characterization of a thyrotropin-releasing hormone deamidase from rat brain. Biochemistry. ,vol. 18, pp. 1206- 1212 ,(1979) , 10.1021/BI00574A014
CHARLES H. EMERSON, WALTER VOGEL, BRUCE L. CURRIE, WITH THE TECHNICAL ASSISTANCE OF TO OKAL, Concentrations of thyrotropin-releasing hormone (TRH) and deamido-TRH, and TRH deamidase activity in brain. Endocrinology. ,vol. 107, pp. 443- 449 ,(1980) , 10.1210/ENDO-107-2-443
C. Oliver, P. Gillioz, P. Giraud, B. Conte-Devolx, Degradation of TRH and its analogues by rat serum and brain homogenate. Biochemical and Biophysical Research Communications. ,vol. 84, pp. 1097- 1102 ,(1978) , 10.1016/0006-291X(78)91696-0
J.R. McDermott, A.I. Smith, J.A. Biggins, M.Chyad Al-Noaemi, J.A. Edwardson, Characterization and determination of neuropeptides by high-performance liquid chromatography and radioimmunoassay Journal of Chromatography B: Biomedical Sciences and Applications. ,vol. 222, pp. 371- 379 ,(1981) , 10.1016/S0378-4347(00)84137-7
G. Boschi, N. Launay, R. Rips, Induction of wet-dog shakes by intracerebral ‘acid’ TRH in rats Neuroscience Letters. ,vol. 16, pp. 209- 212 ,(1980) , 10.1016/0304-3940(80)90346-8