Characterization of cells isolated and cultured from human bone.

摘要: Cells isolated from samples of human iliac crest and femoral heads by collagenase digestion have been successfully cultured in Fitton-Jackson modified BGJb culture medium supplemented with penicillin (100 units/ml), streptomycin micrograms/ml), fetal calf serum (10%). Although only a low proportion the cells survived initial plating (less than 1%), established were readily passaged. Examination obtained at intervals during showed that percentage attached increased time digestion. Rapid sample preparation rat bone did not substantially increase number attaching. Thus, it seems unlikely survival was due to loss viability transportation preparation. Of several media tested 10% supported best growth. Population doubling averaged 104 hr. Cultured assayed for alkaline phosphatase activity using azo dye method naphthol ASTR phosphate as substrate. A portion (19%) demonstrated high all cultures examined regardless passage culture. Autoradiography exposed [3H]thymidine incorporation label into both phosphate-positive -negative cells. The stimulation cell proliferation growth factors studied determining DNA. specific skeletal factor stimulated several-fold half-maximal effect 5 micrograms/ml. Insulin, epidermal factor, crude somatomedin C also proliferation.