Enzymatic mechanisms and detoxification of dry olive-mill residue by Cyclocybe aegerita, Mycetinis alliaceus and Chondrostereum purpureum

摘要: Abstract The detoxification of dry olive-mill residue (DOR), a lignocellulosic by-product, was investigated using three agaric fungi: Cyclocybe aegerita, Mycetinis alliaceus and Chondrostereum purpureum. lignin-modifying enzymes (LME) secretion pattern the above-mentioned fungi such as DyP-type peroxidase (DyP), laccase (Lac), unspecific peroxygenase (UPO), manganese (MnP) determined in presence absence DOR. In C. (Lac) (UPO) induction found when DOR present medium. Thus, these appeared to be responsible for and, indirectly, its plant-growth-promoting effect. experiment performed with M. alliaceus, no differences were (DyP) basal barley medium supplemented However, MnP Lac activities DOR-barley reached maximum after 5 weeks incubation concomitant decline phytotoxicity. purpureum completely eliminated phytotoxicity but significant production LME detected soy or fungal media. Other enzymatic mechanisms also investigated, relation hydrolytic intracellular system cytochrome P450 monooxygenase (CYPs). Our results suggest participation complex (intra extracellular) biotransformation