Association of an abnormality of neutrophil chemotaxis in human periodontal disease with a cell surface protein.

摘要: Localized juvenile periodontitis (LJP) is characterized by severe, early-onset, molar and incisor bone loss; neutrophil chemotaxis disorders; a high prevalence of Actinobacillus actinomycetemcomitans infection. LJP further significant familial aggregation the disease. Recent work in our laboratory has demonstrated selective depletion surface glycoprotein 110,000 Mr (GP110) from neutrophils using labeling with [14C]formaldehyde autofluorography. The function GP110 unknown; however, it does not appear to be chemotactic factor receptor. Rather, bound monoclonal antibody (NCD-1) that recognizes differentiation antigen which itself alters secreting functions. To quantify on normal neutrophils, fluorescein-labeled NCD-1 was amount fluorescence evaluated cytofluorography. Our results indicate there quantifiable reduction (40%) GJP compared controls. Other patients defects express quantities GP110, suggesting disease specificity. data suggest may useful marker for provide probe study dysfunction. Images