Association of Rap1a and Rap1b proteins with late endocytic/phagocytic compartments and Rap2a with the Golgi complex
作者： Marino Zerial , Robert G. Parton , Véronique Pizon , Cecilia Bucci , Michel Desjardins
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摘要: Among the small GTPases of Ras family, Rap proteins exhibit highest homology with p21Ras. The four so far identified constitute two subgroups, comprising Rap1(A,B) and Rap2(A,B) proteins. intracellular location Rap1A, Rap1B Rap2A was investigated in mammalian cells by confocal immunofluorescence microscopy. Using a specific anti-Rap1 affinity-purified antibody, both Rap1A were localized to late endocytic compartments (late endosomes/lysosomes) fibroblasts. localization B transiently overexpressed vaccinia T7 system identical that observed for endogenous Rap1 In contrast, epitope-tagged protein colocalized several markers Golgi complex, thus indicating its site function distinct from Rap1A. addition, morphological subcellular fractionation studies provided evidence association phagosomes displaying biochemical features structures J774 macrophages. Thus, implicates their involvement endocytic/phagocytic processes.
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