Isolation of intact astrocytes from the optic nerve head of adult mice.

摘要: Abstract The astrocytes of the optic nerve head are a specialized subtype white matter that form direct cellular environment unmyelinated ganglion cell axons. Due to their potential involvement in glaucoma, these have become target research. heterogeneity tissue, which also contains other types, some cases it may be desirable conduct gene expression studies on small numbers well-characterized or even individual cells. Here, we describe simple method isolate astrocytes. This permits obtaining with intact morphology from adult mouse and reduces contamination isolated by types. Individual can recognized collected under microscopic control. whole procedure completed 2–3 h. We discuss downstream applications like multiplex single-cell PCR quantitative (qPCR).