T cell epitopes of type II collagen that regulate murine collagen-induced arthritis.

摘要: Chick type II collagen (CII), a protein commonly found in joint cartilage, induces an autoimmune arthritis when administered to susceptible strains of mice. A cyanogen bromide fragment CII, CB11, contains the requisite epitopes critical for inducing collagen-induced arthritis. If as tolerogen, however, before immunization, CB11 prevents onset disease. Therefore, delineation structural elements that can regulate autoreactive T cells became goal this study. To delineate antigenic cells, 14 peptides containing overlapping sequences were generated. Mononuclear from CII-immunized DBA/1 mice cultured with these and resulting supernatants examined production IFN-gamma. Two peptides, CII 181-209 245-270, generated greatest responses. The ability two was tested by administering them neonatal tolerogens immunization CII. Both suppressed incidence whereas no other peptide used tolerogen significantly altered course four arthritis-resistant murine did not recognize either immunized disease-susceptible B10.Q responded well both. Thus, coincidence cell responses 245-270 CIA-susceptible lack response disease-resistant or CII-tolerized identify important CIA.