Vascular Endothelial Cell Growth Factor–Induced Tissue Factor Expression in Endothelial Cells Is Mediated by EGR-1

摘要: Vascular endothelial cell growth factor (VEGF) is a major regulator of angiogenesis. We report here that treatment cells with VEGF leads to upregulation tissue mRNA and protein expression on the surface. Reporter gene studies show transcriptional activation by mediated GC-rich promoter element containing overlapping binding sites for Sp1 EGR-1. As shown immunofluorescence electrophoretic mobility shift assays, upon EGR-1 rapidly accumulates in nucleus binds its respective recognition site promoter. occupies this unstimulated seems be partially displaced increasing amounts Transfection an plasmid mimics transcription observed after treatment. In contrast, NFκB, involved inflammatory stimuli, not activated VEGF. These data induces response largely distinct from suggest mediator possibly other VEGF-responsive genes.