miR-134 in extracellular vesicles reduces triple-negative breast cancer aggression and increases drug sensitivity.

摘要: Exosomes (EVs) have relevance in cell-to-cell communication carrying pro-tumorigenic factors that participate oncogenesis and drug resistance are proposed to potential as self-delivery systems. Advancing on our studies of EVs triple-negative breast cancer, here we more comprehensively analysed isogenic cell line variants their EV populations, tissues well tumour normal tissues. Profiling 384 miRNAs showed miRNA content be highly representative cells origin. most substantially down-regulated aggressive originated from 14q32. Analysis miR-134, the miRNA, supported its clinical tumours compared matched tissue. Functional indicated miR-134 controls STAT5B which, turn, Hsp90. delivered by direct transfection into Hs578Ts(i)8 (in which it was greatly down-regulated) reduced STAT5B, Hsp90, Bcl-2 levels, cellular proliferation, enhanced cisplatin-induced apoptosis. Delivery via miR-134-enriched also migration invasion, sensitivity anti-Hsp90 drugs. While differing effects achieved or delivery likely be, at least partly, due specific amounts these routes, EV-based identified miRNA-134 a biomarker therapeutic for cancer.