Riboflavin synthase genes and enzymes and methods of use

摘要: Through function complementation of E. coli auxotrophs, the ultimate and pentultimate enzymes spinach riboflavin biosynthetic pathway have been cloned, namely, lumazine synthase (LS) (RS). This invention relates to isolation nucleic acid fragments from plants or fungi that encode LS protein. The also RS In addition, construction chimeric genes encoding all a portion LS, in sense antisense orientation, wherein expression gene results production altered levels plant transformed host cell. Furthermore, RS, fungal vivo vitro methods identify herbicide fungicide candidates are included evaluate ability chemical compound inhibit activity enzyme enzyme.