Cryopreservation of the Common Carotid Artery of the Rabbit: Optimization of Dimethyl Sulfoxide Concentration and Cooling Rate

摘要: Abstract This paper describes the continuation of studies that demonstrated suitability CP-Tes solution as a medium for introduction and removal dimethyl sulfoxide in rabbit common carotid arteries established kinetics cryoprotectant permeation tissue. In this we report tolerance artery to sulfoxide, concentrations up 30% (w/w), using technique exposure was designed control osmotic stress. The maximum concentration achieved without damage 15% (w/w). Vessels were then equilibrated with cooled −80 °C at 0.22, 0.69, 2.15, or 9.63 °C/min: they transferred gas phase liquid nitrogen refrigerator {temperature below −160 °C) storage. Thawing carried out 37 °C water bath. optimum rate cooling these conditions found be 0.69 °C/min. maximal recovery contractile force response 10−6 M norepinephrine 30–40%; relaxation acetylcholine (an endothelium-mediated function) 80% control, an estimated 71% endothelial cells survived minimal ultrastructural change.