Supplementing barley or rapeseed meal to dairy cows fed grass-red clover silage: II. Amino acid profile of microbial fractions.

摘要: Four ruminally cannulated dairy cows were used to examine the effect of diet on AA composition rumen bacteria and protozoa, flow microbial nonmicrobial entering omasal canal. Cows offered grass-red clover silage alone, or that supplemented with 5.1 kg DM barley, 1.9 rapeseed meal, barley meal according a 4 x Latin square design 2 factorial arrangement treatments. During first 10 d each period, had free access and, thereafter intake was restricted 95% ad libitum intake. Postruminal digesta assessed using canal sampling technique in combination triple marker method. Liquid- (LAB) particle- (PAB) associated isolated from reticulorumen protozoa Microbial protein determined 15N as marker. Flows similar fed diets meal. However, increased while LAB protozoa. Diet negligible effects profile fractions. Comparison profiles across indicated differences between PAB for out 17 measured. Rumen found be different 14 15 For grass silage-based diets, energy supplementations appear alter postruminal supply through modifications proportionate contribution pools total rather than direct result changes protein.