Impact of routine sanitation on the microbiomes in a fresh produce processing facility.

摘要: Abstract Indigenous bacterial populations in fresh-cut produce processing facilities can have a profound effect on the survival and proliferation of inadvertently contaminating foodborne pathogens. In this study, environmental samples were collected from variety Zone 3 sites plant before after daily routine sanitation. Viable mesophilic aerobic bacteria population was evaluated using both culturing method quantitative real-time PCR (qPCR) propidium monoazide treatment. surface microbiota analyzed 16S rRNA gene amplicon sequencing with Qiime2 bioinformatic pipeline. Over 8000 species across 4 major phyla identified microbiomes facility. Overall, effective reduction observed at sampling production floor, while sanitation peripheral surfaces less evident. Effective resulted qualitive shifts microbiota. Several highly abundant multiple sample for winter summer samplings. Based spatial temporal distribution most species, core microbiome facility tentatively described to included Cupriavidus sp., Pseudomonas Ralstonia Arthrobacter psychrolactophilus, veronii, Stenotrophomonas an unknown family Enterobacteriaceae.