摘要: A carbon paste electrode biosensor for D-lactic acid analysis in biological samples was developed. D-lactate cytochrome c oxidoreductase from baker's yeast (EC 1.1.2.4) used as biorecognition element of the in combination with phenazine methosulphate (PMS)-Reinecke's salt precipitate as mediator. The enzyme bound on due to Enzacryl using glutardialdehyde. response addition was linear concentration range 0.015-100 mM. Limit of detection equal 56 mu M when signal vs. noise criterium (S/N=3) is considered. Specificity only was proven. Relative standard deviation 6.6 % 12 mM D-lactate found. exerts long-term stability and also good stability during repeated measuring cycles. The electrode has a potential use practical samples.
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