The Binding of Kirromycin to Elongation Factor Tu
作者： Alfred PINGOUD , Claus URBANKE , Heinz WOLF , Guenter MAASS
DOI: 10.1111/J.1432-1033.1978.TB12294.X
关键词:
摘要: The influence of kirromycin on the elongation factor Tu (EF-Tu) in its binary and ternary complexes was investigated. equilibrium constant for binding antibiotic to EF-Tu · GDP GTP determined by circular dichroism titrations be 4 × 106 M−1, aa-tRNA a combination hydrolysis protection experiments 2 M−1. In presence aminoacyl-tRNAs is weakened two. changes conformation complex such way that aminoacyl moiety aminoacyl-tRNA more accessible non-enzymatic hydrolysis. It concluded this structural alteration responsible inhibitory action antibiotic.
sci-hub.se 参考文章(11)
Zähner H, Wolf H, Metabolic products of microorganisms. 99. Kirromycin Archives of Microbiology. ,vol. 83, pp. 147- ,(1972)
G E Wilson, M Cohn, Magnetic resonance studies of the manganese guanosine di- and triphosphate complexes with elongation factor Tu. Journal of Biological Chemistry. ,vol. 252, pp. 2004- 2009 ,(1977) , 10.1016/S0021-9258(18)71856-5
Shigeo OHTA, Mamoru NAKANISHI, Masamichi TSUBOI, Ken-ichi ARAI, Yoshito KAZIRO, Structural fluctuation of the polypeptide-chain elongation factor Tu. A comparison of factors from Escherichia coli and Thermus thermophilus HB8. FEBS Journal. ,vol. 78, pp. 599- 608 ,(1977) , 10.1111/J.1432-1033.1977.TB11773.X
Alfred PINGOUD, Detlev BOEHME, Detlev RIESNER, Reinhard KOWNATZKI, Gunter MAASS, Anti‐Cooperative Binding of Two tRNATyr Molecules to Tyrosyl‐tRNA Synthetase from Escherichia coli FEBS Journal. ,vol. 56, pp. 617- 622 ,(1975) , 10.1111/J.1432-1033.1975.TB02269.X
Heinz WOLF, Gianni CHINALI, Andrea PARMEGGIANI, Mechanism of the Inhibition of Protein Synthesis by Kirromycin FEBS Journal. ,vol. 75, pp. 67- 75 ,(1977) , 10.1111/J.1432-1033.1977.TB11504.X
Gianni CHINALI, Heinz WOLF, Andrea PARMEGGIANI, Effect of Kirromycin on Elongation Factor Tu FEBS Journal. ,vol. 75, pp. 55- 65 ,(1977) , 10.1111/J.1432-1033.1977.TB11503.X
T. Blumenthal, J. Douglass, D. Smith, Conformational alteration of protein synthesis elongation factor EF-Tu by EF-Ts and by kirromycin. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 74, pp. 3264- 3267 ,(1977) , 10.1073/PNAS.74.8.3264
Yee-Hsiung Chen, Jen Tsi Yang, Hugo M. Martinez, Determination of the secondary structures of proteins by circular dichroism and optical rotatory dispersion. Biochemistry. ,vol. 11, pp. 4120- 4131 ,(1972) , 10.1021/BI00772A015
Alfred PINGOUD, Claus URBANKE, Gerhard KRAUSS, Frens PETERS, Guenter MAASS, Ternary complex formation between elongation factor Tu, GTP and aminoacyl-tRNA: an equilibrium study. FEBS Journal. ,vol. 78, pp. 403- 409 ,(1977) , 10.1111/J.1432-1033.1977.TB11752.X
H. Hess, Thermo‐chemische Untersuchungen Annalen der Physik. ,vol. 132, pp. 463- 479 ,(1842) , 10.1002/ANDP.18421320713