Serine Specific Transfer Ribonucleic Acids
作者： H. G. Zachau
DOI: 10.1111/J.1432-1033.1968.TB00406.X
关键词:
摘要: Aggregates of two and more tRNASer molecules are formed from purified tRNASeryeast under commonly used storage conditions. The aggregates can also be produced easily by adjusting to pH 3.0 a solution tRNASer, Ser-tRNASer, or unfractionated tRNA subsequently adding neutral Mg++-containing buffer. various were characterized Sephadex elution profiles the rates dissociation at elevated temperatures. At given temperature, EDTA phenol increase rate dissociation. found accept serine conditions enzymatic charging monomer. is lower level only 1/3 1/2 one, which reached with same aggregate sample after prior assay. Since control experiments aim explain incorporation as an artefact (enzyme bound radioactivity; via monomeric tRNA) negative, idea direct was accepted.
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