A new carboxylation reaction. The vitamin K-dependent incorporation of H-14-CO3- into prothrombin.

摘要: The bovine plasma zymogen prothrombin contains a number of gamma-carboxyglutamic acid residues which are not found in an abnormal produced when cattle given the vitamin K antagonist dicoumarol. These modified glutamic appear to be formed post-translationally by reaction requires K. It has been shown that postmitochondrial supernates from K-deficient rats incorporate added H-14-CO3- minus into microsomal proteins upon addition This incorporation is dependent presence precursor preparations, and factors present postmicrosomal supernatant. Most radioactive protein can obtained pellet extraction with 0.25% Triton X-100 identified as it all radioactivity amino-terminal activation fragment prothrombin. portion previously contain residues. Hydrolysis purified resulted loss 50% subsequent chromatography amino hydrolyzate demonstrated remaining was entirely acid. results consistent hypothesis incorporated carboxyl groups