Histology of ovaries of female rabbits immunized with deglycosylated zona pellucida macromolecules of pigs.

摘要: Female rabbits (n = 36, 6 per group) were immunized with: (i) solubilized isolated porcine zona pellucida (SIZP), which contains ZP1, 82 kDa; ZP3 alpha, 55 and beta, (ii) a purified preparation of alpha beta (ZP3); (iii) endo-beta-galactosidase digested glycoproteins alpha-(EBGD) (iv) beta-(EBGD) (each about 30% deglycosylated); (v) chemically deglycosylated core proteins alpha-(DG) (vi) beta-DG greater than 92% deglycosylated). Rabbits injected with saline 6) or Freund's adjuvant served as controls. bled weekly to monitor titres. Every six weeks two animals from each group 16) selected for unilateral oophorectomy followed by histological examination. Sections scored numbers primary, secondary tertiary follicles. Anti-ZP3 titres developed in all treatment groups correlated carbohydrate content (peak cent [125I]-labelled binding radioimmunoassay: SIZP 71.9 +/- 1.2, 70.0 2.5, alpha-EBGD 60.9 5.3, beta-EBGD 56.4 5.0, alpha-DG 4.0, 53.5 4.3) (means SEM). Animals SIZP, showed statistically significant reduction the number follicles compared controls (P less 0.01, MANOVA), whereas alpha-EBGD, did not 0.05, MANOVA). These results demonstrate that immunization macromolecules (ZP3 alpha-DG) does produce histopathological changes ovaries. Such ZP represent potential target antigens immunocontraceptive development.