Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States.

作者: Jennifer Harcourt , Azaibi Tamin , Xiaoyan Lu , Shifaq Kamili , Senthil K. Sakthivel

DOI: 10.3201/EID2606.200516

关键词:

摘要: The etiologic agent of an outbreak pneumonia in Wuhan, China, was identified as severe acute respiratory syndrome coronavirus 2 January 2020. A patient the United States given a diagnosis infection with this virus by state Washington and US Centers for Disease Control Prevention on 20, We isolated from nasopharyngeal oropharyngeal specimens characterized viral sequence, replication properties, cell culture tropism. found that replicates to high titer Vero-CCL81 cells Vero E6 absence trypsin. also deposited into repositories, making it broadly available public health research communities. hope open access reagent will expedite development medical countermeasures.

参考文章(24)
A. Stieneke-Gröber, M. Vey, H. Angliker, E. Shaw, G. Thomas, C. Roberts, H.D. Klenk, W. Garten, Influenza virus hemagglutinin with multibasic cleavage site is activated by furin, a subtilisin-like endoprotease. The EMBO Journal. ,vol. 11, pp. 2407- 2414 ,(1992) , 10.1002/J.1460-2075.1992.TB05305.X
Wenhui Li, Michael J. Moore, Natalya Vasilieva, Jianhua Sui, Swee Kee Wong, Michael A. Berne, Mohan Somasundaran, John L. Sullivan, Katherine Luzuriaga, Thomas C. Greenough, Hyeryun Choe, Michael Farzan, Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature. ,vol. 426, pp. 450- 454 ,(2003) , 10.1038/NATURE02145
J. T. Simpson, K. Wong, S. D. Jackman, J. E. Schein, S. J.M. Jones, I. Birol, ABySS: A parallel assembler for short read sequence data Genome Research. ,vol. 19, pp. 1117- 1123 ,(2009) , 10.1101/GR.089532.108
Dipankar Das, Mavanur R. Suresh, Copious production of SARS-CoV nucleocapsid protein employing codon optimized synthetic gene Journal of Virological Methods. ,vol. 137, pp. 343- 346 ,(2006) , 10.1016/J.JVIROMET.2006.06.029
T. Scobey, B. L. Yount, A. C. Sims, E. F. Donaldson, S. S. Agnihothram, V. D. Menachery, R. L. Graham, J. Swanstrom, P. F. Bove, J. D. Kim, S. Grego, S. H. Randell, R. S. Baric, Reverse genetics with a full-length infectious cDNA of the Middle East respiratory syndrome coronavirus Proceedings of the National Academy of Sciences. ,vol. 110, pp. 16157- 16162 ,(2013) , 10.1073/PNAS.1311542110
Laurence Josset, Vineet D. Menachery, Lisa E. Gralinski, Sudhakar Agnihothram, Pavel Sova, Victoria S. Carter, Boyd L. Yount, Rachel L. Graham, Ralph S. Baric, Michael G. Katze, Cell Host Response to Infection with Novel Human Coronavirus EMC Predicts Potential Antivirals and Important Differences with SARS Coronavirus mBio. ,vol. 4, ,(2013) , 10.1128/MBIO.00165-13
James T Robinson, Helga Thorvaldsdóttir, Wendy Winckler, Mitchell Guttman, Eric S Lander, Gad Getz, Jill P Mesirov, Integrative genomics viewer Nature Biotechnology. ,vol. 29, pp. 24- 26 ,(2011) , 10.1038/NBT.1754
A. C. Sims, S. C. Tilton, V. D. Menachery, L. E. Gralinski, A. Schafer, M. M. Matzke, B.-J. M. Webb-Robertson, J. Chang, M. L. Luna, C. E. Long, A. K. Shukla, A. R. Bankhead, S. E. Burkett, G. Zornetzer, C.-T. K. Tseng, T. O. Metz, R. Pickles, S. McWeeney, R. D. Smith, M. G. Katze, K. M. Waters, R. S. Baric, Release of Severe Acute Respiratory Syndrome Coronavirus Nuclear Import Block Enhances Host Transcription in Human Lung Cells Journal of Virology. ,vol. 87, pp. 3885- 3902 ,(2013) , 10.1128/JVI.02520-12
David E. Swayne, David L. Suarez, Erica Spackman, Terrence M. Tumpey, Joan R. Beck, Dean Erdman, Pierre E. Rollin, Thomas G. Ksiazek, Domestic poultry and SARS coronavirus, southern China. Emerging Infectious Diseases. ,vol. 10, pp. 914- 916 ,(2004) , 10.3201/EID1005.030827
Matthew Kaye, Julian Druce, Thomas Tran, Renata Kostecki, Doris Chibo, Jessica Morris, Mike Catton, Chris Birch, SARS–associated Coronavirus Replication in Cell Lines Emerging Infectious Diseases. ,vol. 12, pp. 128- 133 ,(2006) , 10.3201/EID1201.050496