The Maize-Microbe Bioassay: A Unique Approach to Environmental Mutagenesis
作者： James M. Gentile , Michael J. Plewa
DOI: 10.1007/978-1-4613-3566-5_8
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摘要: A plant-microbe bioassay was devised to test agricultural chemicals for mutagenic activity under conditions that are normally encountered in the environment. Maize plants, homallelic waxy mutation [wx/wx], were grown field isolation plots and each plot sprayed with a known concentration of given chemical. Additional plants greenhouse treated like manner. Plants harvested at various stages development, tissues homogenized, filtered, centrifuged 1,000 g give soluble fraction which tested Salmonella typhimurium Saccharomyces cerevisiae. Controls consisted extracts from untreated plants. This system offers distinct advantages because mutagenicity any plant-mediated metabolite can be detected, genetic effect chemical itself maize plant by back reversion analysis wx locus. Positive data obtained on several chemicals, particular, members s-triazine herbicides, nonmutagenic classical Salmonella/mammalian microsome assay. Further, tissue found capable activiting well-documented promutagens, including aflatoxin B1.
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