Effects of torsion on intervertebral disc gene expression and biomechanics, using a rat tail model.

摘要: Study design In vitro and in vivo rat tail model to assess effects of torsion on intervertebral disc biomechanics gene expression. Objective Investigate promoting biosynthesis producing injury caudal discs. Summary background data Torsion is an important loading mode the increased torsional range motion associated with clinical symptoms from disruption. Altered elastin content implicated degeneration, but its are unknown. Although compression have been studied, effect expression Methods biomechanical tests were performed segments subjected 4 treatments: elastase, collagenase, genipin, control. rats Ilizarov-type fixators implanted five 90 minute groups: 1 Hz cyclic ± 5 15° 30°, static + sham. Anulus nucleus tissues separately analyzed using qRT-PCR for anabolic, catabolic, proinflammatory cytokine markers. Results showed decreased stiffness following elastase treatment no changes frequency. significant dynamic time. Cyclic upregulated anulus fibrosus. Up regulation TNF-α IL-1β was measured at ±30°. Conclusion We conclude that strong differences response apparent increasing resulting a more substantial increase metabolism pulposus. highlight importance suggest remodels shearing. Torsional can cause excessive amplitudes detectable biologically before they biomechanically.