Heat shock response in Bombyx mori cells infected by nuclear polyhedrosis virus (NPV)
作者： M. B. Evgen'ev , T. Y. Braude-Zolotareva , E. A. Titarenko , A. V. Levin , O. N. Denisenko
DOI: 10.1007/BF00339736
关键词:
摘要: Silkworm (Bombyx mori) cells are characterized by the ability to synthesize heat shock proteins (hsps) at exceptionally high temperatures (up 48° C). We infected B. mori larvae and tissue culture with nuclear polyhedrosis virus (Mn NPV) studied response in cells. Our results indicate that not able hsps after temperature elevation, polyhedrin (the major inclusion body protein of Mn being practically only translational product such Similar were obtained for fifth instar mori. On other hand Northern hybridization experiments employing Drosophila melanogaster hsp70 gene coupled data on vitro RNA transcription showed elevation induces active mRNAs Therefore, discrimination favour viral mRNA probably takes place level translation.
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sci-hub.se 参考文章(12)
Ulrich K. Laemmli, Rodney A. Johnson, Maturation of the head of bacteriophage T4. II. Head-related, aberrant tau-particles. Journal of Molecular Biology. ,vol. 80, pp. 601- 611 ,(1973) , 10.1016/0022-2836(73)90199-X
L. G. Davis, W. M. Kuehl, J. F. Battey, Basic methods in molecular biology ,(1986)
Joseph R. Nevins, Induction of the synthesis of a 70,000 dalton mammalian heat shock protein by the adenovirus E1A gene product Cell. ,vol. 29, pp. 913- 919 ,(1982) , 10.1016/0092-8674(82)90453-6
J.José Bonner, Mary Lou Pardue, The effect of heat shock on RNA synthesis in Drosophila tissues. Cell. ,vol. 8, pp. 43- 50 ,(1976) , 10.1016/0092-8674(76)90183-5
T. Ya. Braude-Zolotarjova, V. T. Kakpakov, N. G. Schuppe, Male diploid embryonic cell line of Drosophila virilis In Vitro Cellular & Developmental Biology – Plant. ,vol. 22, pp. 481- 484 ,(1986) , 10.1007/BF02623449
William M. BONNER, Ronald A. LASKEY, A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels FEBS Journal. ,vol. 46, pp. 83- 88 ,(1974) , 10.1111/J.1432-1033.1974.TB03599.X
Matthew P. Scott, Jennifer M. Fostel, Mary Lou Pardue, A new type of virus from cultured Drosophila cells: Characterization and use in studies of the heat-shock response Cell. ,vol. 22, pp. 929- 941 ,(1980) , 10.1016/0092-8674(80)90570-X
Susumu Maeda, Takashi Kawai, Masuo Obinata, Hiroyuki Fujiwara, Tadashi Horiuchi, Yoshiyuki Saeki, Yoshinari Sato, Mitsuru Furusawa, Production of human α -interferon in silkworm using a baculovirus vector Nature. ,vol. 315, pp. 592- 594 ,(1985) , 10.1038/315592A0
Robert Holmgren, Kenneth Livak, Richard Morimoto, Robert Freund, Matthew Meselson, None, Studies of cloned sequences from four Drosophila heat shock loci. Cell. ,vol. 18, pp. 1359- 1370 ,(1979) , 10.1016/0092-8674(79)90246-0
Patricia Z. O'Farrell, Howard M. Goodman, Patrick H. O'Farrell, High resolution two-dimensional electrophoresis of basic as well as acidic proteins Cell. ,vol. 12, pp. 1133- 1142 ,(1977) , 10.1016/0092-8674(77)90176-3