Novel quantitative assays for estimating the antimicrobial activity of fresh garlic juice.

摘要: Novel agar diffusion and broth dilution assays were developed for quantitatively estimating the antimicrobial activity of fresh garlic juice. Bacteria found to be inhibited by juice in assay included two gram-positive five gram-negative species. Leuconostoc mesenteroides was not inhibited. Escherichia coli B-103 (HB101, with pJH101, ampicillin resistant, 100 microg ml(-1)) chosen as standard culture quantitative assays. The based on slope ratio method, where dose response divided methylmethane thiosulfonate (MMTSO2). Juice from varied between 1.76 2.31 MMTSO2 per mg decreased during 11 months storage cloves at 5 degrees C less than 0.1 also used E. culture, which permitted selective enumeration this bacterium when ml(-1) incorporated into enumerating agar. Selective essential since sterile and, thus, contained natural flora. Growth unaffected 0.1%, delayed 0.25%, completely 0.5 2% 24 h incubation 37 micro C. minimum inhibition concentration was, estimated 0.5%, is equivalent 3.46 assay.