Characterization of the Aminotransferase ThdN from Thienodolin Biosynthesis in Streptomyces albogriseolus.
作者： Daniela Milbredt , Eugenio P. Patallo , Karl-Heinz van Pée
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摘要: In Streptomyces albogriseolus the indolethiophen alkaloid thienodolin is derived from tryptophan. The first step in biosynthesis regioselective chlorination of tryptophan 6-position indole ring. second catalyzed by aminotransferase ThdN. ThdN shows sequence homology (up to 69 % similarity) with known pyridoxal 5′-phosphate-dependent aminotransferases aspartate family Gram-positive bacteria. thdN was heterologously expressed Pseudomonas fluorescens, and enzyme purified nickel-affinity chromatography. a homodimeric mass 90 600 kDa catalyzes conversion l-tryptophan number chlorinated brominated l-tryptophans. lowest KM values were found for 6-bromo- 6-chlorotryptophan (40 66 μm, respectively). For it 454 μm, which explains why major product dechlorothienodolin only minor component. turnover (kcat) 7-chlorotryptophan (128 min−1) higher than that natural substrate (88 min−1).
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