Preparation, characterization and biodistribution of ultrafine chitosan nanoparticles.

摘要: Abstract Chitosan nanoparticles cross-linked with glutaraldehyde have been prepared in AOT/n hexane reverse micellar system. The cross-linking the polymeric network has confirmed from FTIR data. Because of adhesive nature these particles, their sizes, as measured by QELS, found dependent on particle density aqueous buffer. size also to vary amount cross-linking. actual chitosan a particular degree determined at infinite dilution particles water. is ≈30 nm diameter, when 10% amine groups chains and it shoots up 110 diameter all are (100% cross-linked). TEM pictures show that spherical shape remain form aggregation. biodistribution after intravenous injections mice showed readily evade RES system blood for considerable time. γ image rabbit administration 99mTechnetium (99mTc) tagged confirms above observation, pool visible even 2 h. picture shows distribution heart, liver, kidneys, bladder vertebral column. Interestingly, studies indicated distributed bone marrow also, implying possibility using imaging targeting purpose.