Inhibition of proinsulin to insulin conversion in rat islets using arginine and lysine analogs. Lack of effect on rate of release of modified products.

摘要: Rat pancreatic islets were exposed to a combination of analogs arginine (3 mM canavanine) and lysine thialysine) for 2 h then labeled with [3H]leucine in the continued presence analogs. Control incubated parallel without Prelabeled 3-h chase period Incorporation blocked conversion newly synthesized, radioactive, proinsulin insulin. No untoward toxic effects found on nonradioactive insulin as measured by radioimmunoassay. The rate release modified was no different from that synthesized absence and, such, susceptible normal action enzymes responsible conversion. These results confirm previously untested hypothesis beta-granule is minimal functional unit release; granules are thus handled B-cell released at which independent physicochemical nature their contents.