Rapid Identification of Emerging Pathogens: Coronavirus

摘要: We describe a new approach for infectious disease surveillance that facilitates rapid identification of known and emerging pathogens. The process uses broad-range polymerase chain reaction (PCR) to amplify nucleic acid targets from large groupings organisms, electrospray ionization mass spectrometry accurate measurements PCR products, base composition signature analysis identify organisms in sample. demonstrate this principle by using 14 isolates 9 diverse Coronavirus spp., including the severe acute respiratory syndrome–associated coronavirus (SARS-CoV). show method could distinguish between SARS other CoV, human CoV 229E OC43, individually mixture all 3 viruses. sensitivity detection, measured titered SARS-CoV spiked into serum, was ≈1 PFU/mL. This approach, applicable bacterial, viral, fungal, or protozoal pathogens, is capable automated >900 reactions per day.