Antimalarial activity of extracts and fractions from Bidens pilosa and other Bidens species (Asteraceae) correlated with the presence of acetylene and flavonoid compounds

摘要: After interviewing natives and migrants from the Amazon region of Brazil about plants traditionally used for treatment malaria fever and/or liver disorders, we selected identified 41 different species, including native Bidens (Asteraceae). We have undertaken an antimalarial study pilosa other species abroad. The crude ethanol extracts (whole plant, leaves roots) chloroform butanol fractions B. at concentrations 50 microg/ml caused up to 90% inhibition Plasmodium falciparum growth in vitro. In vivo partial reduction berghei parasitemia mice. nine collected outside were tested, seven inhibited parasite vitro by 65-91%. As appears be a promising agent, further characterized substances responsible such activity. HPLC analysis using photo diode-array detector showed phenyl acetylene flavonoids extract roots. roots, which 86% vitro, contained major component as 1-phenyl-1,3-diyn-5-en-7-ol-acetate. association activity presence compounds is discussed. summary, all had aliphatic acetylenes (6-14 each) also very active, whereas parriflora bitternata with none or three acetylenes, respectively reported literature, inactive borderline