Fundamentals of protein interaction network mapping

摘要: Studying protein interaction networks of all proteins in an organism ("interactomes") remains one the major challenges modern biomedicine. Such information is crucial to understanding cellular pathways and developing effective therapies for treatment human diseases. Over past two decades, diverse biochemical, genetic, cell biological methods have been developed map interactomes. In this review, we highlight basic principles interactome mapping. Specifically, discuss strengths weaknesses individual assays, how select a method appropriate problem being studied, provide general guidelines carrying out necessary follow-up analyses. addition, computational predict, map, visualize interactomes, summary some most important resources. We hope that review serves as both useful overview field guide help more scientists actively employ these powerful approaches their research.