Genome-Wide Reprogramming of Transcript Architecture by Temperature Specifies the Developmental States of the Human Pathogen Histoplasma.

摘要: Eukaryotic cells integrate layers of gene regulation to coordinate complex cellular processes; however, mechanisms post-transcriptional remain poorly studied. The human fungal pathogen Histoplasma capsulatum (Hc) responds environmental or host temperature by initiating unique transcriptional programs specify multicellular (hyphae) unicellular (yeast) developmental states that function in infectivity pathogenesis, respectively. Here we used recent advances next-generation sequencing uncover a novel re-programming transcript length between Hc cell types. We found ~2% percent transcripts exhibit 5’ leader sequences differ markedly morphogenetic states. Ribosome density and mRNA abundance measurements differential revealed nuanced translational regulation. One such class regulated longer exhibited tight repression. Further examination these dually repressed genes some control morphology their strict is necessary for the make appropriate decisions response temperature.