Physiological Characterization of a Bacterial Consortium Reductively Dechlorinating 1,2,3- and 1,2,4-Trichlorobenzene

摘要: A bacterial mixed culture reductively dechlorinating trichlorobenzenes was established in a defined, synthetic mineral medium without any complex additions and with pyruvate as the carbon energy source. The maintained over 39 consecutive transfers of small inocula into fresh media, enriching activity. In situ probing fluorescence-labeled rRNA-targeted oligonucleotide probes revealed that two major subpopulations within microbial consortium were phylogenetically affiliated sublineage Desulfovibrionaceae gamma subclass Proteobacteria. grew by fermentation pyruvate, forming acetate, propionate, CO2, formate, hydrogen. Acetate propionate supported neither reduction nor sulfate when present. Hydrogen formate used for to sulfide. Sulfate strongly inhibited reductive dechlorination trichlorobenzenes. However, depleted due reduction, started. Similar results obtained sulfite present cultures. Molybdate at concentration 1 mM Cultures supplied molybdate plus did not reduce sulfate, but occurred. Supplementation electron-depleted cultures various electron sources demonstrated direct donor dechlorination, whereas hydrogen not.