Plasma protein binding in uremia: Extraction and characterization of an inhibitor

摘要: Plasma protein binding in uremia: Extraction and characterization of an inhibitor. The impairment drugs other substances to serum albumin patients with uremia can be restored normal or near levels by adsorption charcoal synthetic polymers at pH 3. We used a nonionic poly-styrene-divinylbenzene copolymer treat uremic plasma observed marked improvement binding. Subsequent elution this resin ethanol produced substance that, when dried recombined plasma, caused dose-dependent phenytoin tryptophan Restoration affinity occurred after retreatment abnormalized pleural fluid exudate from yielded, extraction the above technique, inhibitor(s) amounts averaging five times that extracted equal volumes plasma. This inhibitor (I x ) is water soluble, heat stable, dialyzable across cellophane membranes. Unlike fatty acids, which also interfere binding, I partitions primarily phase solvent partition studies but undergoes sharp transition 4 5 range, suggesting presence carboxyl group. These findings lend further support hypothesis retained ligand(s) responsible for impaired associated suggests role organic acids known accumulate renal failure. Liaison aux proteines plasmatiques dans l'uremie: et caracterisation d'un inhibiteur. L'alteration de la liaison drogues d'autres l'albumine serique au cours l'uremie peut etre completement ou presque supprimee par l'adsorption sur du charbon des polymeres synthetiques Nous avons utilise un co-polymere non ionique polystyrenedivinylbenzene pour traiter le uremique 3 observe une amelioration importante liaison. L'elution ulterieure cette resine l'ethanol produit qui, lorsqu'elle est sechee recombinee avec normal, determine alteration dose dependante diphenyl-hydantoine tryptophane. La recuperation d'une affinite normale ete obtenue apres nouveau traitement Le liquide malades uremiques donne, technique ci-dessus, inhibiteur(s) phenylhydantoine en quantite cinq fois plus grande que celle extraite devolumes identiques normal. Cet inhibiteur soluble l'eau, thermostable dialysable travers membranes cellophane. A difference acides gras, qui peuvent aussi interferer liaison, passe aqueuse etudes solvants, mais subit brusque gamme 5, ce suggere groupe carboxyle. Ces constatations apportent arguments supplementaires l'hypothese selon laquelle ligand (ou ligands) retenus responsable l'alteration plasmatique organiques dont l'accumulation connue l'insuffisance renale.