Plasma Membrane Synthesis in the Macrophage following Phagocytosis of Polystyrene Latex Particles

摘要: Phagocytosis of polystyrene latex beads by mouse peritoneal macrophages results in the interiorization large amounts plasma membrane to form phagolysosomal membrane. The isolated phagolysosomes is initially similar cholesterol and phospholipid content, carries 5′-nucleotidase activity with characteristics marker enzyme. Immediately after ingestion, amount has descreased as judged a reduction cell volume, spreading, pinocytosis, phagocytosis. During next 6 hours, decreases half-life about 2 hours. Total cellular also degree accounted for decrease enzyme proportion interiorized. After lag levels constituents begin increase. final net increase linearly proportional ingested Only at this time do spread out on glass surface, resume regain ability phagocytize new test particle. reaching control 10 12 hours This not associated phagolysosomes. These changes suggest synthesis replace interiorized during requires presence exogenous molecules use In absence cholesterol, functions are restored does return levels. inhibition protein RNA phagocytosis blocks increment phospholipid, activity.